Hi,
In <Pine.A41.4.21.0010051321080.89938-100000 at homer06.u.washington.edu>
at 5 Oct 2000 21:25:33 +0100, 'Mike' Michael J. Moser wrote:
>Andrej
>>There is a paper from Roger Tsiens lab where they fused two
>spectral variants of GFP to calmodulin. The resulting molecule was able
>to serve as a measure of intracellular Ca2+ by FRET. See:
>>Nature 1997 Aug 28;388(6645):882-7 Related Articles, Books, LinkOut
>>Fluorescent indicators for Ca2+ based on green fluorescent proteins and
>calmodulin.
>>Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, Ikura M, Tsien RY
>>Happy GFPing,
>>Mike Moser, Ph.D.
>Department of Pathology, University of Washington
>Health Sciences Building, 1959 N.E. Pacific, Room K-065
>Box 357705, Seattle, WA 98195-7705
>Tel: 206.543.6585
>FAX: 206.543.3967
>moser at u.washington.edu>http://faculty.washington.edu/moser>>On Thu, 5 Oct 2000, Andrej.Michalik wrote:
>>> Dear netters,
>> I wonder whether anyone ever produced GFP (or EGFP) fused to a protein at
>> each end - "internal GFP", and whether it stayed fluorescent? Is it
>> trial and error or should I better not try it?
>> Thanx
>> Andrej
>>>>>>>> ------------------------------------------------------------------------------
>> A andrej michalik
>> A A department of biochemistry
>> A A university of antwerp (uia)
>> A A universiteitsplein 1
>> AAAAAAAAAAAAA B-2610 antwerpen
>> A AAA belgium
>> A A tel: +32-(0)3-8202642
>>amichal at uia.ac.be>> ------------------------------------------------------------------------------
>>>>>>>>>>>>>>>>>>>>---
----
John Doe
