IUBio

antibody -fluorescence

Malini Viswanathan malini at helix.mgh.harvard.edu
Fri Nov 17 14:48:06 EST 2000


	I'm having a problem with quenching data - i have quenching data
for an entire IgG, while that of the same antibody that was expressed in
bacteria (now a Fab) and purified does not show equivalent quenching. Is
there any reference that addresses this problem? Has anyone had this
experience before? Any suggestions/pointers would be appreciated.

Thanks in advance

Malini






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