Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Thu Oct 5 05:57:01 EST 2000

Kathryn Sunn <k.sunn at garvan.unsw.edu.au> wrote:
> Has anyone tried double labelling with EGFP, are they relative in brightness?

In my experience, EGFP is much brighter than Ds-Red when illuminated
with either an argon-krypton laser or a Hg lamp. In the confocal, you
can obviously correct for this.

As for your problem, the first thing to check would be the transfection
efficiency. Next I would transfect cells with unfused Ds-Red vector to
find out whether you have problems with your microscope.


/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */

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