GFP tagged on both sides

'Mike' Michael J. Moser moser at u.washington.edu
Thu Oct 5 15:25:33 EST 2000


There is a paper from Roger Tsiens lab where they fused two
spectral variants of GFP to calmodulin.  The resulting molecule was able
to serve as a measure of intracellular Ca2+ by FRET.  See:

Nature 1997 Aug 28;388(6645):882-7 Related Articles, Books, LinkOut 

Fluorescent indicators for Ca2+ based on green fluorescent proteins and

Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, Ikura M, Tsien RY

Happy GFPing,

Mike Moser, Ph.D.
Department of Pathology, University of Washington                  
Health Sciences Building, 1959 N.E. Pacific, Room K-065
Box 357705, Seattle, WA  98195-7705
Tel: 206.543.6585
FAX: 206.543.3967
moser at u.washington.edu

On Thu, 5 Oct 2000, Andrej.Michalik wrote:

> Dear netters,
> I wonder whether anyone ever produced GFP (or EGFP) fused to a protein at
> each end - "internal GFP", and whether it stayed fluorescent? Is it
> trial and error or should I better not try it?
> Thanx
> Andrej
> ------------------------------------------------------------------------------
>         A               andrej michalik              
>        A A              department of biochemistry 
>       A   A             university of antwerp (uia)
>      A     A            universiteitsplein 1
>     AAAAAAAAAAAAA       B-2610 antwerpen  
>    A         AAA        belgium
>   A           A         tel: +32-(0)3-8202642    
>                         amichal at uia.ac.be
> ------------------------------------------------------------------------------


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