FLUORESCENT-PROTEINS: Kozac sequence in XFP-N vectors

Birgitta Olofsson olofsson at lebs.cnrs-gif.fr
Fri Oct 13 11:42:49 EST 2000

What do you think about the fact that Clontech have added a Kozac consensus
CCACC sequence just before the ATG of the enhanced fluorescent protein and
after the MCS in EGFP-N and EYFP-N vectors? I suppose that in order to
obtain a fusion protein without internal traduction of the fluorescent
protein by itself we have to take away this Kozac stuff. Have anyone of you
used these plasmids with the Kozac sequence still in place?
I will be very happy to get an answer from you.


Birgitta Olofsson, Directeur de Recherche CNRS
Laboratoire d'Enzymologie et Biochimie Structurales - LEBS
Avenue de la Terrasse, Bat. 34
Tel:(33-1) 69 82 35 07  Fax: (33-1) 69 82 31 29
e-mail: birgitta.olofsson at lebs.cnrs-gif.fr


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