In article <Pine.GSO.4.21.0010052104170.16130-100000 at hgems>,
"Andrej.Michalik" <amichal at uia.ua.ac.be> wrote:
> Dear netters,
> I wonder whether anyone ever produced GFP (or EGFP) fused to a protein at
> each end - "internal GFP", and whether it stayed fluorescent? Is it
> trial and error or should I better not try it?
> Thanx
> Andrej
>> ------------------------------------------------------------------------------
> A andrej michalik
> A A department of biochemistry
> A A university of antwerp (uia)
> A A universiteitsplein 1
> AAAAAAAAAAAAA B-2610 antwerpen
> A AAA belgium
> A A tel: +32-(0)3-8202642
>amichal at uia.ac.be> ------------------------------------------------------------------------------
>
Check out fig 1, JCB 148(3), 505-517 (2000). Also seem to recall people
using concatenated GFPs to increase signal with some success-GFP flanked
by GFPs. As always, try it and see.
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