IUBio

GFP and secretion

Dominic-Luc Webb molmed domweb at mbox.ki.se
Fri Feb 9 12:51:11 EST 2001


On 29 Jan 2001, Sébastien Paris wrote:

> Hi GFPers,
> I've constructed a vector expressing GFP with a signal peptide of secretion, in S. cerevisea.
> I would like to know if GFP, after extracellular secretion, could be fluorescent and if yes, if it should be observed directly under microscope ?
> Thanks

Yes, assuming it doesn't get degraded.

Cheers,


Dominic

North 59 37' 30"
East  17 48' 10"

_______________________________________

Dominic-Luc Webb, doktorand


Lab:
Department of Molecular Medicine
Endocrinology and Diabetes Unit
Rolf Luft Center for Diabetes Research
Karolinska Hospital L3
S-17176 Stockholm
Sweden
Tel: Int+46-8-517-74829
Fax: Int+46-8-517-79450

Home:
Tingvallav. 88, 1 tr
195 32 Märsta
Sweden
Tel/Data/Fax: Int+46-8-591-27121 (UNIX dialin server)
Internet Email: dominic at enk.ks.se
________________________________________






More information about the Fluorpro mailing list

Send comments to us at biosci-help [At] net.bio.net