Fixation of cells for FACS and storage

Marcus Watkins mwatkins at cyllene.uwa.edu.au
Tue Jan 16 19:14:48 EST 2001


I am hoping to fix EGFP expressing cell culture lines for FACS analysis
and store them until I can analyse them. By the looking through the
archive it looks like paraformaldehyde fixation is the best, but how
long can I store cells in this solution?

Basically I'd like to know what is the best fixation technique and how
long I can store cells with the minimum loss of fluorescence.  



"If at first you don't succeed, try, try, again. Then quit. There's no
use in being a damn fool about it." --W.C. Fields

Marcus Watkins
Postgraduate Student
Centre For Applied Cancer Studies/Lions Cancer Institute
University of Western Australia


More information about the Fluorpro mailing list

Send comments to us at biosci-help [At] net.bio.net