IUBio

GFPuv Western Blot

Rainbow kbsb7s04 at hkem.com
Tue Jan 30 00:32:58 EST 2001


Dear all,

Recently I have expressed my GFPuv protein from clontech in E. coli TOP10F'. I could detected a band corresponding to 27kDa when I did Western blot. However, I also found an additional band which is around 50kDa, even though when I repeat the experiment again.

Did anyone has the similar experience. Thanks.

Kenneth
Email: kbsb7s04 at hkem.com
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