Dear all,
I have some problems with the isolation of GFP from infected plant tissue. I
want to use GFP in pathogenicity assays, this in order to determine the degree
of disease. I work on a plant pathogen, Botrytis cinerea, a pathogen that
causes rot of plant tissue. I have constructed a fluorescent transformant that
is still fully pathogenic. However, I cannot find any fluorescence in planta.
I did most of the control experiments and it turns out that there is a factor
X that blocks GFP fluorescence. Mixing a fluorescent sample with an extract
from infected plant tissue blocks fluorescence within seconds. This factor is
not present in an extract from noninfected tissue. It is not low pH since
strong buffering does not help.
Since this is a typical "needle in the haystack" I am open for any
suggestions.
Thank you!
Arjen ten Have
Laboratory of Phytopathology
Wageningen University
Binnenhaven 9
PO Box 8025
6700 EE Wageningen
The Netherlands
Phone: +31-317483126
Fax: +31-317483412
Email: arjen.tenhave at fyto.dpw.wau.nl
Internet:http://www.dpw.wau.nl/fyto/,http://www.dpw.wau.nl/fyto/arjen.htm
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