while I am well fixed tissue (rat-pup brain) for
vibratome sectioning, 4% PFA fixation for
immunohistochemistry is yielding poorly fixed tissue
even with over night fixation.
a. use 4% PFA in PBS instead of 4% PFA in
b. increase the concentration of PFA as the
powder is quite old.
Is their some other alternative.
Dr. Sunil Thomas,
National institute of mental health and neurosciences,
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