Dear all,
I want to buy Hoechst 33342 to stain my living COS-7 cells. (DAPI staining
does not work).
Is there anybody having experience with this dye? Molecular Probes does not
provide a
protocol for nuclei staining. Which concentrations do I use? What is the
incubation time?
Any problems or tricks with this fluorescent marker? Do you think the
Hoechst Dye is
a good marker?
Thank you in advance,
Ulrich Huth
Ulrich.huth at pharmazie.uni-freiburg.de
=====================================
Ulrich Huth
Albert-Ludwigs-Universitaet
Pharmazeutische Technologie
Stefan-Meier-Str.19
79104 Freiburg
Germany
Tel: 0761-203 4899
Fax: 0761-203 6326