Short Course in Protein Purification Using GFP

Daniel Gonzalez meton at rci.rutgers.edu
Sun Jun 30 22:09:32 EST 2002

I have been asked to announce the following,

                  The State University of New Jersey
                  Rutgers (Campus at New Brunswick)

             Center for Research and Education in Bioluminescence
                          and Biotechnology
                   Programs in Biotechnology, Presents:

Protein Purification: Isolation, Analysis, and Characterization of GFP,
A Five and One-Half Day Hands-On Laboratory Course Using the remarkable
Green-Fluorescent Protein (GFP), A Novel Marker For Gene Expression,
as the source material;

                               July 21-26, 2002
                                August 4-9, 2002
                             January 12-17, 2003
                           and  March 16-21, 2003

More than 1100 scientists from around the world have strongly
this intensive course as an opportunity to develop protein research and
analytical skills in a retreat setting. Participants work hard, identify

and solve problems in the lab and enjoy camaraderie and good food and
beer with colleagues.  An added bonus of this course is practical
laboratory exposure to the GFP, now a staple of life sciences
laboratories everywhere.

This five and one-half day laboratory course covers a wide variety of
conventional methods for protein isolation, purification, and
characterization.  The course format integrates hands-on laboratory
exercises with classroom lectures, demonstrations, study breaks, and
short take-home assignments.

A special feature of the course is that all laboratory work will be
performed on the same starting sample (native or recombinant Aequorea
GFP), which will be purified from an exceedingly crude form to near
homogeneity as judged by high performance liquid chromatography
(HPLC), SDS gel electrophoresis, isoelectric focusing, and capillary
zone electrophoresis. This feature provides a continuity of purpose,
integrating dozens of preparative and analytical protein techniques in
a way that few competing courses can match.

A problem-solving approach will be used throughout the course.  Under
the guidance of experienced lab instructors, participants will work in
groups of three to plan their own protocols, analyze data, and interpret

results. A student-teacher ratio not greater than 8:1 will be maintained

and the faculty coordinators will be present throughout the course.

For further details you can reach us,

by E-mail at:  meton at rci.rutgers.edu or crebb at rci.rutgers.edu
by phone at:  (732) 932-9071 extension 225 or 216
by FAX at:  (732) 932-3633

for a brochure and further information please visit the GFP purification

short course official Web site at:

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