Hello,
I was hoping someone could help me - I have made GFP constructs and
transfected them into my cells. After sorting, the FACS means range
from about 80 -100 (with a negative of about 3-4). However, when I take
these cells to the fluorescence microscope, I do not see any
fluorescence. I have been fixing my cells with 4% formaldehyde,
followed by methanol treatment. I then add a 1:1 solution of
PBS:glycerol, and seal with molten agarose. I visualize using the FITC
filter on our microscope.
Any suggestions why I do not see anything would be greatly appreciated.
Thanks.
