hello,
I used the first generation of DsRed (dsRed1) vectors and had difficulties
of solubility with the protein produced (agregation). So, I can't accuratly
quantify the Dsred protein in a western analysis. Moreover, the DsRed
protein is not commercially available and the DsRed antibody has no
cross-reactivity with the GFP. I'm searching for a relatively pure quantity
of dsRed protein (to do my western blot) or for a solution to enhance the
solubility of my protein (I have tested ionic pH modifications but no
change) . If somebody could help me . Thanks Corinne
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