Dear Jos,
I came across your question on GFP and using a vital red fluorescent dye
for DNA staining on the web. Did you get anywhere with all this? I am
also looking into using an alternate to H33342 and GFP, your help will
be greatly appreciated.
kind regards
Huseyin
_________________________________________________________________
Dear reader,
Currently I am examining living cells with Green Fluorescent Protein
and I want to combine this with a red fluorescent dye to visualize DNA
in vital cells using confocal laser scanning microscopy. I cannot use
Hoechst or other dyes with UV excitation, since cells will be damaged
and since our laser cannot excite Hoechst. Does anyone know of a vital
red fluorescent DNA dye with absorption spectrum around 567 nm and
emission above 600 nm? I already tried several dyes from Molecular
Probes (Syto-17 and Syto-64) but they appear to stain no nuclear DNA in
living eukaryotic cells.
Thank you very much for your reply.
Jos Broers
--
Huseyin Sumer Ph.D.
NH&MRC Peter Doherty Biomedical Research Fellow
Centre For Reproduction and Development
Monash Institute of Medical Research
Mailing Address:
Level 3
Centre For Reproduction and Development
Monash Institute of Medical Research
Monash Medical Centre
246 Clayton Road
Clayton, Vic 3168
e-mail: huseyin.sumer at med.monash.edu.au
Ph +61 3 9594 7100 or +61 3 9594 7359 (direct)
Fax +61 3 9594 7311
http://www.monashinstitute.org/
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