Hi,
Im a final year sudent in university and am currently finishing my project
on synchronisation of plant cell suspension cultures. Im preforming a transformation
using Arabidopsis cells and and Agrobacterium strains ACD2 and GDR. My supervisor
said I was to test for transformation using GFP. I did this ana my cells
did fluoresce a green colour which I understand to be a positive for transformation.
What Im not clear about is whether the strains express GFP and this is why
they fluoresce. Can anyone help me with this?
Thanks
Anita
