Greetings GenBank Users,
GenBank Release 122.0 is now available via ftp from the National Center
for Biotechnology Information:
Ftp Site Directory Contents
---------------- --------- ---------------------------------------
ncbi.nlm.nih.gov genbank GenBank Release 122.0 flatfiles
ncbi-asn1 ASN.1 data used to create Release 122.0
Uncompressed, the Release 122.0 flatfiles require roughly 42698 MB
(sequence files only) or 47573 MB (including the 'index' files). The
ASN.1 version requires roughly 38419 MB. From the release notes:
Release Date Base Pairs Entries
121 Dec 2000 11101066288 10106023
122 Feb 2001 11720120326 10896781
Close-of-data was 02/13/2001. Seven business days were required to prepare
this release. In the eight-week period between close-of-data for GenBank 121.0
and GenBank 122.0, GenBank grew by 0.619 billion basepairs and 790,758
sequence records, and the number of human bases broke the 7 Gbp threshold.
We would like to remind our users that a GenBank mirror site is
available at ftp://genbank.sdsc.edu/pub . Please consider using this site
in order to speed up your transfer of GenBank releases.
For additional release information, see the README files in either of the
directories mentioned above, and the release notes (gbrel.txt) in the
genbank directory. Sections 1.3 and 1.4 of the release notes (Changes in
Release 122.0 and Upcoming Changes) have been appended below.
Release 122.0 data are currently available via NCBI's Entrez and Blast
servers, and the 'query' email server.
New GenBank cumulative update files (gbcu.flat.Z and gbcu.aso.Z), containing
only those entries new/updated since the Release 122.0 close-of-data, should be
available by 07:00am EST, February 24. Please note that the new CUs will be
smaller than previous versions you might have obtained after Release 121.0 was
posted.
If you encounter problems while ftp'ing or uncompressing Release 122.0,
please send email outlining your difficulties to info at ncbi.nlm.nih.gov .
Mark Cavanaugh, Vladimir Alekseyev, Anton Butanaev
GenBank
NCBI/NLM/NIH
1.3 Important Changes in Release 122.0
1.3.1 Organizational changes
Due to database growth, the EST division is now being split into 106 pieces.
Due to database growth, the GSS division is now being split into 34 pieces.
Due to database growth, the HTG division is now being split into 25 pieces.
Due to database growth, the STS division is now being split into 3 pieces.
1.3.2 Alternative GenBank FTP site
A mirror of the GenBank FTP site at the NCBI is available from the San Diego
Supercomputer Center:
ftp://genbank.sdsc.edu/pub
Some users who experience slow FTP transfers of large files (entire releases,
the
GenBank Cumulative Update, etc) might find an improvement in transfer rates from
this alternate site when traffic at the NCBI is high.
1.4 Upcoming Changes
1.4.1 New HTC division to be introduced
A new GenBank division for unfinished high-throughput cDNA sequencing (HTC)
will be included in GenBank releases starting in April 2001 (Release 123.0).
HTC sequences may have 5'UTR and 3'UTR at their ends, partial coding regions,
and introns. A keyword of "HTC" will be present, in addition to division code
"HTC". Those HTC sequences that undergo finishing (eg, re-sequencing) will move
to the appropriate taxonomic GenBank division and the "HTC" keyword will be
removed. A recent project that generates HTC-quality data is described in:
Hayashizaki, Y.
Functional annotation of a full-length mouse cDNA collection
Nature 409, 685-690 (2001)
1.4.2 Minor change to REFERENCE line
The REFERENCE keyword for the literature citations associated with a GenBank
record currently requires a parenthetical component indicating either the
basepair span to which the citation applies, or "sites" for citations providing
annotation rather than sequence data. Here are some examples:
REFERENCE 1 (bases 1 to 262290)
REFERENCE 2 (sites)
REFERENCE 3 (bases 1 to 456; bases 700 to 2334)
In some cases, sequence updates provided by submittors can involve a large
number of changes. And sometimes, a submittor does not wish to indicate
exactly _which_ basepair spans are involved. Accordingly, we will change the
definition of the REFERENCE line to make the parenthetical component an
optional element as of GenBank Release 123.0 (April 2001).
1.4.3 NCBI's ftp address will be changed
At some point in the near future NCBI's ftp address will be changed.
The current address:
ncbi.nlm.nih.gov
will become:
ftp.ncbi.nih.gov
Additional details about this change will be made available via these
release notes and the GenBank newsgroup (bionet.molbio.genbank) as they
become available.
1.4.4 Selenocysteine representation
Selenocysteine residues within the protein translations of coding
region features have been represented in GenBank via the letter 'X'
and a /transl_except qualifier. At the May 1999 DDBJ/EMBL/GenBank
collaborative meeting, it was learned that IUPAC plans to adopt the
letter 'U' for selenocysteine.
DDBJ, EMBL, and GenBank will thus use this new amino acid abbreviation
for its /translation qualifiers. Although a timetable for its appearance
has not been finalized, we are mentioning this now because the introduction
of a new residue abbreviation is a fairly fundamental change.
Details about the use of 'U' will be made available via these release
notes and the GenBank newsgroup as they become available.
1.4.5 New REFERENCE type for on-line journals
Agreement was reached at the May 1999 collaborative DDBJ/EMBL/GenBank
meeting that an effort should be made to accomodate references which are
published only on-line. Until specifications for such references are
available from library organizations, GenBank will present them in a manner
like this:
REFERENCE 1 (bases 1 to 2858)
AUTHORS Smith, J.
TITLE Cloning and expression of a phospholipase gene
JOURNAL Online Publication
REMARK Online-Journal-name; Article Identifier; URL
This format is still tentative; additional information about this new
reference type will be made available via these release notes.
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