In article <92231.161618FORSDYKE at QUCDN.QueensU.CA> <FORSDYKE at QUCDN.QueensU.CA> writes:
>>> Yes. So, what determines the concentration of self proteins in a cell?
>> Yes. So what determines the concentration of viral proteins in a cell?
Synthesis - Turnover would give us the number of copies of protein X
>>> Under what conditions would the virus be in "excess"?
>Depends on the virus. Picorna viruses "take over" the cell's protein
synthesis machinery and make gobs of viral proteins. However these infections
are lytic, and the infected cell may not have loaded class I sufficiently
to be a target before it is lysed. Other viruses don't make as much viral
proteins, especially those that, at a point in their life cycle, are in the
proviral form. A single viral protein would be in molar xs of any other
single cellular protein when that v-protein is required in large copy
numbers for packaging the viral nucleic acid. As I mentioned before, an
example may be the viral capsid protein. If a capsid is made of aggregted
protein X, and the infected cell will produce a large number of virions, then
I assume that protein X would be in molar xs of any other single protein,
cellular of viral. Goerge Chacko indicated earlier that homotypic adhesion
was dependent on protein concentration, pH, and the notorious "other factors."
Since we realy can't say for certain which proteins agggregate better at
cellular pH, and how the "other factors" play a role, we seem to be left with
[protein] as the only thing that we can grasp.
Are there any virologists out there who can give examples of which viral
proteins may in in large xs. Seems that someone should have tried a
Comassie stain on PAGE'd lysates of infected cells, only to find a big, blue
blob that was absent from the uninfected cells, yet dominated the infected-cell
lane on the gel.
> Sincerely,
> Don Forsdyke
>>References: Forsdyke, D. (1992) Bionet.immunology 812 947edt
> Prasad, S. (1992) Bionet.immunology 814 1516gmt
> Forsdyke, D. (1992) Bionet.immunology 817 1757edt
> Prasad, S. (1992) Bionet.immunology 818 133gmt
Shiv
