IUBio

Hybridoma problems

brian at bio.aau.dk brian at bio.aau.dk
Tue Jul 21 10:25:30 EST 1992


Hello out there!

I am working in a protein lab (an therefor I do not have much knowledge
about immunology). We have in collaboration with another group been 
succesful in producing some specific polyclonal antibodies. Therefore
we wanted to continue to make monoclonal antibodies.
Our fusion was ok, it was between the spleen cells and X63, the 
hybridomas were proliferation and a test showed that they were also
producing antibodies against our protein.

BUT!!! Then we passaged the hybridomas, and after this they are no longer
able to proliferate.

WHY?  has anyone expierence with the same thing, and especially can anyone
give any suggestions, which might help us overcome this problem.

Peter Kristensen
Aarhus University
Department of Chemistry
Division of Biostructural Chemistry
Denmark

E-mail : brian at bio.aau.dk



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