In article <1992Jul21.162530.298 at bio.aau.dk> brian at bio.aau.dk writes:
>>BUT!!! Then we passaged the hybridomas, and after this they are no longer
>able to proliferate.
>>WHY? has anyone expierence with the same thing, and especially can anyone
>give any suggestions, which might help us overcome this problem.
There can be thousands of possible reasons.
The most common ones are:
1. Enough FBS?
2. Very low initial cell concentration?
Give us some more feedback.
Gregory Dandulakis
Biochemical Engineering Lab
U. of Virginia
