In article <2div26$6hv at netnews.upenn.edu> cychan at mail.sas.upenn.edu
(Cecilia Y Chan) writes:
>>I am having a lot of problems with fading using flourescein label for Ab.
>I have tried various mounting reagents, but they all seem to fade
[stuff deleted]
You do not say what you have tried, so excuse me if this is redundant...
We have had good luck with three formulations:
1) Commercial media such as FluorSave (Calbiochem) or whatever the name of
Molecular Probes' similar product is (Slow Fade, I think). (I have no
connection to either of these companies.)
2) A product from Mallinckrodt diagnostics division called Conray (sold as
a contrast agent for X-rays, I think).
3) Homemade formulas containing n-propyl-gallate in buffered glycerol (I can
send references/recipes to anyone interested).
Have you tried any of these? If so, I'm stumped!
Jim
--
Jim Hutchins [] E-Mail: jbh at anat.umsmed.edu
Asst Prof of Anatomy [] Asst Prof of Neurology
Univ Mississippi Med Ctr [] Jackson, MS
