: > >I have a hybridoma cell line contaminated with fibroblasts.
: > >Is there any way of selectively getting rid of these fibroblasts?
: > >
: > >Thanks for any help and comments,
: > >Chris R.
I posted the definitive solution last week, or at least I thought
I did.
Question: How were the hybridomas separated from all the others that made
the wrong Ab in the first place?
Answer: They were cloned!!
Re-clone your line by limiting dilution. This is a good practice anyway to
keep your line expressing high levels of Ab. Consult a hybridoma manual for
cloning protocols. Also, I would pop some of your cells into mice to make
ascites--this will allow you to make buckets of Ab and probably get rid of the
fibroblasts as well.
Jerry Woodward
University of Kentucky
