Hello,
This is a call for help to anyone who has ever used a myeloid cancer cell line of the murine type named C1498. Has anyone ever done any surface phenotype by FACS to confirm that this is a myeloid tumor cell line. You see, the only positive phenotype i get is CD13+. All my other phenotypes are negative (CD14-, CD33-, CD34-, esterase negative, peroxydase negative, Sudan black negative) and so i have this problem.... I need either a second phenotype to confirm C1498 to be myeloid or any histochemical test other than the ones mentioned. Can anyone point me to a myeloid marker other than the ones I've done???
any help would be appreciated,
Stephane C. Pion
PIONS at ERE.UMontreal.CA
:w
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