In article <C7n45r.F56 at news.cis.umn.edu>, shiv at hiv.med.umn.edu (Shiv A.
Prasad) wrote:
>> Does anyone know about a column which depletes Con A from Con A supernatants?
> I've seen this in a Materials&Methods somewhere, but can't remember where :-(
> Shiv A. Prasad shiv at lenti.med.umn.edu> Dept. of Microbiology pras0005 at student.tc.umn.edu> Univ. of Minnesota
I've never heard about solid phase depletion of Con A from supts, but we
regularly add alpha-methyl-mannoside (20mM final:2ug/ml Con A) to supts
which contain Con A. It effectively neutralizes all of Con A bioactivity.
I don't have the ref handy but if you e-mail me, I can dig around a little.
Hope this helps!
Allen Black]
medm003 at uabdpo.dpo.uab.edu
Univ. of Alabama at Birmingham
Dept. of Micro
