brett at BORCIM.WUSTL.EDU wrote:
: Hey- I'm trying to use a mAb obtained from an ATCC hybridoma as ascites fluid
: in a Western blot. So far I have had no luck, although the epitope should be
: recognized in denatured form as the antibody was raised against a peptide. IS
: there a way to search the literature for the use of this antibody in a western?
: Offhand, does anyone have any experience using CRL-1729 (anti c-myc)?
: I am...much obliged
: Brett Lindenbach
Brett.
Did you try running a search through CD-ROM / Medline using:
(CRL-1729 OR c-myc) AND (western OR blot* or Immunoprecipit* or membran*) ???
You might get lucky... :-)
Hope this helps, Benny.
**************************************************************
* Benny Shomer *
* Tel-Aviv University *
* Sackler School of Medicine, Dept.of Embryology and Teratology*
*--------------------------------------------------------------*
* Snail: Ramat-Aviv , Tel-Aviv 69978 , Israel. *
* E-mail : pc386 at ccsg.tau.ac.il *
* Tel : 972-3-640-9238 FAX : 972-3-642-2046 *
* *
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