European Collection of Animal Cell Cultures (ECACC) via Gopher

[Dan Jacobson]

You can now access data from the European Collection of Animal Cell
Cultures (ECACC) data via Gopher.

Point your gopher client at gopher.gdb.org (merlot.welch.jhu.edu will
still work for a while but we are switching over) and select:


12. Search Databases at Hopkins (Vectors, Promoters, NRL-3D, EST, OMIM./

   -->  6.  Culture Collections (ATCC, ECACC)/

    -->  2.  ECACC - European Collection of Animal Cell Cultures/



and you'll see:


 -->  1.  Information from and about the ECACC/
      2.  Cell Lines <?>
      3.  DNA Probes <?>
      4.  HLA-defined Human B-Lymphoblastoid Cell Lines <?>
      5.  Hybridomas <?>


Here you will find a number databases which you can search in order 
to find strains, cell lines or recombinant materials of interest as 
well as information about the ECACC, and ordering and deposit information. 


To search for Cell Lines select:

 -->  2.  Cell Lines <?>

and enter a word or series of words that describes your interests.  You
will then see a list of articles that have at least one of the words you
typed in.


You can further modify your search by use of the words 'and' and 'not'.
For example:

human and fibroblast

Will select articles which contain both the word 'human' and the word
'fibroblast'. 


This pulls up quite a few entries so you can add on a few more
search terms to narrow it down further-

human and fibroblast and dna and repair

You may also use wildcards (*) to search for partial words.  If you
wanted to search for the words - mutate, mutated, mutates, mutation,
mutations ... you would simply use:

mutat*


Typical entries retrieved from these databases are:


 -->  2.  Cell Lines <?> :

----------------------------------------------

            ECACC number 90011801
                Cell name 1BR3
         Cell Description Human skin fibroblast
           Culture medium EMEM (EBSS) + 1% NEAA + 15% FBS
           Passage Number 5
       Subculture routine Split confluent cultures 1:2 to 1:4 ie seeding
                         at 3-5x10,000 cells/cm} using 0.25% trypsin
                          or trypsin/EDTA; 5% CO2; 37xC
               Morphology Fibroblast
 Research council deposit Yes
                Depositor Dr C Arlett, MRC Cell Mutation Unit, University
                          of Sussex
                Reference Int J Radiation Biol 1988;54:911; Nature 1975;258:427
                 Comments Derived from a skin biopsy of a normal adult
                          male.  The cells have been used in cell
                          survival and mutation studies
             Applications DNA repair and cell growth studies

----------------------------------------------



-->  3.  DNA Probes <?> :

----------------------------------------------


             ECACC number P92022101
               Clone name H9-13
                   Origin Rabbit
            Gene Fragment Oxysterol binding protein
                   Vector pUC13
              Insert Size 1.48
                 DNA Type cDNA
                Depositor Dr. Paul A. Dawson
                          Wake Forest University
                          Bowman Gray School of Medecine
                          Department of International Medecine
                          300 South Hauthorne Road
                          Winston
                          North Carolina 27103
                          USA

----------------------------------------------



-->  4.  HLA-defined Human B-Lymphoblastoid Cell Lines <?> :


----------------------------------------------


             ECACC number 86021105
                Cell name BAE
                  Markers A2, B51, DR8, DQ4, DW8
          WorkShop number 9W0807
                   Origin GSW
                          Professor Hans Grosse-Wilde
                          Institut fur Immungenetik
                          Universitatsklinikum Essen
                          Virchowstrasse 171, D-4300 Essen 1
                          FEDERAL REPUBLIC OF GERMANY
 
----------------------------------------------



-->  5.  Hybridomas <?> :


----------------------------------------------

             ECACC number 92012403
                Cell name ER25
              Description Human T cell clone
                   Medium RPMI 1640 + 10% Hu S + supplements
         Immunocyte donor Human peripheral blood lymphocytes
          Transfected DNA Not applicable
                Immunogen Mixture of APD and BSM cells (both in-vitro
                          EBV transformed lymphoblastoid cell lines
               Reactivity Human TcR gamma/delta clone (will not cause
                          lysis of Daudi cells)
       Subculture routine The cells are cultured in 96-round bottomed
                          microtitre plates. The culture period is
                          approximately 7 days; 5% CO2; 37xC
                Receptors CD2/CD3/CD11a/CD18/CD25/CD26
                Depositor Dr E Sturm, The Doctor Daniel Den Hoed Cancer
                          Center, Rotterdam, The Netherlands
                Reference J Immunol 1990;145:3202
                 Comments The human TcR gamma/delta clone expresses
                          a V delta 1 encoded TcR. It does not lyse Daudi cells
             Applications Basic research/collaborative only
 Research Council deposit No

----------------------------------------------


If you've never heard of gopher just write me a note and I'll send you
some information to help get you started.


Happy searching,

Dan Jacobson

danj at welchgate.welch.jhu.edu

Johns Hopkins University