On 21 Apr 1994, Shahram Mori wrote:
> [ Article crossposted from bionet.molbio.methds-reagnts ]
> [ Author was Shahram Mori ]
> [ Posted on 21 Apr 1994 17:14:23 GMT ]
>>> Dear netters,
> We have 11 ug of a protein isolated from polyacrylamide gel
> electrophoresis. We are interested in isolating the protein from the gel
> and making antibodies to it. We would very much appreciate if you had any
> input and specially references that would allow us to isolate the protein
> effieciently in order to use for antibody production.
> Thanks a milion for your help.
> cheers.
> Shahram Mori
> Program in Molecular Biology
> Dept. of Chemistry Box 3C
> NMSU Las Cruces NM
> 88003
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>>>Dear Shahran
No need to isolate the protein from the gel. You can blot the protein,
stain, take small strips of the nitrocellulose and implant into the
spleen of an animal (rat, mouse, rabbit). You will obtain hight titles of
antiody. (Ref. Ove Nilsson et al. J. Immunol. Methods 1987, 99:67-75)
Good Luck!!
M. Mariano
