I am posting this for a friend who does not have an Internet access. He
had been working for about two years in the lab next to mine. Tigran is
one of the brightest young scientist I ever met, and a most amiable
person. Being in Russia (or Armenia, for that matter) he is wasting his
talent. This is a real shame.
He is looking for a postdoctoral, or a similar position in cell biology
or immunology, including clinical immunology. If interested, you can try
and contact him directly. If this proves difficult, reply to my address.
Sincerely,
Andrey
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
* Dr Andrey Tsouladze * *
* Department of Biology * You *
* Technion - Israel Institute of Technology * have *
* Haifa 32000 * been *
* Israel * warned... *
* E-mail: tsoul at aluf.technion.ac.il * *
* E-mail: tsoul at techunix.technion.ac.il * *
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
CURRICULUM VITAE
Tigran K. DAVTYAN
Address: Laboratory of Immunology
Institute of Epidemiology, Virology, and
Medical Parasitology
Republic of Armenia Ministry of Health
21a Gevork Kochar
375009 Yerevan, Armenia
Phone: +7 8852 56 21 02
Telex: 243318 ARAKS SU
Fax: +7 8852 56 40 30
Born: December 1, 1966, Yerevan, Armenia
Family: Married, my wife: Asmik Arakelyan, born October
7, 1966, Engineer in Physics
We have two daughters: Araks, born May 19,
1989, and Arusyak, born September 29, 1992
Citizenship: Armenian
Highest degree: Ph.D. (Candidate of Biol. Sci.)
1993
Research Interests: Molecular mechanisms of stress resistance and
immunity, autoimmunity; molecular mechanisms of
antigen presentation and antibody production,
genetic regulation of immune response
Education:
1988 Graduated from Department of Pharmacology and
Medical Chemistry, Yerevan Medical Institute
Professional Background:
1988 - present Research scientist in the Laboratory of
Molecular and Cellular Immunology, Institute of
Epidemiology, Virology, and Medical
Parasitology, Armenia Ministry of Health.
We work there on the in vitro immunization of
human peripheral blood lymphocytes for getting
human-mouse heterohybridomas which produce
human monoclonal antibodies against bacterial
toxin and viral proteins. We have obtained some
antibody0producing clones to these antigens
which appeared to be rather unstable in respect
to their karyology and human immunoglobulin
production.
1988 - 1989 Senior technician in the Laboratory of
Molecular and Cellular Immunology, Institute of
Molecular Biology, Acad.Sci.Armenia.
During this period, I had been working on
establishing mouse hybridomas.
1989 - 1992 Research scientist, Laboratory of Genetic
Mechanisms of Cell Malignization and
Differentiation headed by Dr T.N.Ignatova,
Institute of Cytology, St.-Petersburg, Russia.
During my stay in this institute, I was
involved in the project of multiple drug
resistance of hybridoma and myeloma cells.
Cells selected for ethidium bromide resistance
were shown to produce more immunoglobulins and
have their MDR1 gene amplified. Both selected
and unselected cells synthesize and secrete
much more immunoglobulins when treated with
EtBr or adriamycin. Adriamycin stimulates the
synthesis of immunoglobulins via free radical
production of hybridoma cells.
Also, we have shown that these drugs bind
immunoglobulins inside and outside the cell.
Analogous data were obtained using normal human
lymphocytes. These data made me state that
there exist an additional mechanism (different
from MDR-mediated) of drug efflux from the cell
via immunoglobulin secretion.
1993 I have got my Ph.D. in Genetics and Immunology
at the Dept of Genetics and Cytology of Yerevan
University. It is entitled "Induction of Immune
Response of Human lymphocytes in vitro". The
supervisors of my Ph.D. work are: Corresponding
Member of the Natl.Acad.Sci.Armenia, Director
of the Institute of Epidemiology, virology, and
Medical parasitology (Yerevan), Prof Yuri T.
Alexanyan, and Head of the Laboratory of
Genetic Mechanisms of Cell Malignization and
Differentiation of the Institute of Cytology,
Acad.Sci.Russia (St.-Petersburg), Prof Tatyana
N. Ignatova.
Methods: Various variants of immunoenzyme assay,
immunoblotting, gel electrophoresis, isolation
and purification of DNA, RNA, and proteins from
cells, human cells separation, cultivation,
hybridoma generation, and production of
antibodies.
PUBLICATIONS
1. T.K.Davtyan, B.Ch.Nisman, Yu.T.Alexanyan, and T.N.Ignatova (1991).
The possibility of producing the human monoclonal antibody by
immunization of human peripheral blood lymphocytes in vitro. Biol. J.
Armenia 44, No.2, p.115.
2. M.B.Melixetyan, T.K.Davtyan, E.V.Beriozkina, T.N.Ignatova, and Yu.T.
Alexanyan (1991). The effect of adriamycin and ethidium bromide on
the antibody production by mouse B-cell hybridomas. Biol. J. Armenia
44, No.2, p. 120.
3. T.K.Davtyan, G.I.Blinova, Yu.T.Alexanyan, and T.N.Ignatova (1991).
Induction of long-term proliferation of normal human lymphocytes by
co-cultivation with ethidium bromide resistant L929 lines of mouse
transformed fibroblasts. Biol. J. Armenia 44, No.2, p. 155.
4. T.K.Davtyan, B.Ch.Nisman, T.N.Ignatova, and Yu.T.Alexanyan (1991).
The effect of L-leucin methyl ester on the functional activity of
human peripheral blood lymphocytes in vitro. Biol. J. Armenia 44,
No.3, p. 235.
5. Yu.T.Alexanyan, and T.K.Davtyan (1991). The problem of producing
human monoclonal antibodies. Immunologia, No.3, p. 10.
6. T.K.Davtyan, M.B.Melixetyan, T.N.Ignatova, and Yu.T.Alexanyan (1993).
Adaptive response of B-cells to cytotoxic drugs in culture. 1.
Adriamycin and ethidium bromide stimulate the synthesis and secretion
of immunoglobulins by hybridomas and lymphocytes. Tsitologia 35, No.
4, p. 54.
7. T.K.Davtyan, M.B.Melixetyan, Yu.T.Alexanyan, and T.N.Ignatova (1993).
Adaptive response of B-cells to cytotoxic drugs in culture. 2. The
interaction of adriamycin and ethidium bromide with immunoglobulin
molecules. Tsitologia 35, No.6/7, p. 91.
8. M.B.Melixetyan, T.K.Davtyan, I.V.Ivanova, Yu.T.Alexanyan, and
T.N.Ignatova (1993). Adaptive response of B-cells to cytotoxic drugs
in culture. 3. A possible mechanism of adriamycin action on
immunoglobulin production by mouse hybridoma cells. Tsitologia 35,
No.6/7, p.98.
9. T.K.Davtyan, T.B.Smirnova, Yu.T.Alexanyan, T.N.Ignatova, and M.B.
Melixetyan (1993). The interaction of cultivated human peripheral
blood lymphocytes with mouse transformed fibroblasts. Immunologia,
No.3, p.17.
10.T.K.Davtyan (1993). Induction of Immune Response of Human lymphocytes
in vitro. Ph.D. thesis. Dept of Genetics and Cytology. Yerevan
University.
