Hi.
I have a question for those using any of the fusion protein expression
systems such as GST and the like. We are interested in setting this
technology up in our lab. Our goal is not only to make the fusion peptides
as a source of purified peptide by cleaving off the GST but also for use in
immunization, and thatt's where my question lies.
Ig GST is kept on the fusion peptide by eluting it off the column, can the
GST serve as the carrier in immunizations, circumventing the necessity of
coupling the peptide to a more traditional carrier like KLH?
I know this can be done using the pRIT-2T vector from Pharmacia, but I have
read in the literature (and heard from the director of the local hybridoma
facility) that immune response directed against fusion protiens carrying
protein-A often have the immune response biased toward the Protein-A and
not the peptide. Is this phenomena been observed using GST or MBP based
vectors?
Thanks in advance,
David
(also crossposted (I hope) on the bionet.immunology sub)
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