help

[Ctro Inmunologia Molecular]

   Hi Immunonetters:
 
   I am biochemist and I have some experience with antigen 
 
   quantification by mean of ELISA methods. This type of assays has a 
 
   good reproducibility, but assays for detection of autoantibodies are 
 
   very weak and in some cases the background values are more higher 
 
   than the test values. I will appreciate any suggestion in this sense, 
 
   what kind of blocking is the best and a general schedule of some 
 
   particular system that somebody have been setup for this purpose.
 
   Thanks in advance.....
 
 
   Gilda Nunez.  
 
   monoclon at infomed.sld.cu