I cloned a glycoprotein in a cell line and have antibodies against its
sugar moiety. I do immunofluorescence and the protein is there, then I
perform an immunoblot with the same Ab and nothing stains. I arrived to load
exctracts of 10,000,000 cell on SDS PAGE- still nothing. I strongly confide
in the expertiseof of somebody of you out there, dear colleagues.
Sincerely
Giorgio
