In article <33qh4p$fvd at arcturus.ciril.fr>,
Gilbert FAURE <faure at grip.u-nancy.fr> wrote:
>Who has experience with the assessment of human basophils from whole blood or
>enriched suspensions, using flow cytometry? We cannot find them on the
>scattergrams!!!
>>Thank you for your help.
>>Gilbert C Faure
Greetings, Gilbert,
I did some basophil studies on a flow cytometer about two years ago.
I did this work at a corporate lab, so I wasn't allowed to keep my notebooks,
and the information that I will be giving you I will be reconstructing from
memory.
As I recall, basophils appear in almost exactly the same spot as
monocytes on a forward scatter/side scatter dot plot. It's impossible to
distinguish the basophils on the basis of light scatter alone. They are
relatively rare - about 1% of the total leukocyte count. However, using
labeled IgE or anti-IgE antibody, one can distinguish basophils by fluor-
escence. Sorry, I don't remember who makes the reagents, but they are
commercially available.
Good luck!
--
Unique ID : Ladasky, John Joseph Jr.
Title : BA Biochemistry, U.C. Berkeley, 1989
Location : Stanford University Dept. of Cell Biology, Fairchild D-105
Keywords : immunology, music, running, Green
