In article <3d6non$50v at mserv1.dl.ac.uk>, "RUIZ VAZQUEZ, Elena" <ervazquez at mail1.cti.unav.es> says:
>>(second version, the first one may be walking through the cyberspace...)
>>I performed a human PBL culture (one hundred, actually).This culture was
>performed in order to test immuno activity of a peptide called Pa. After
>culturing (RPMIc, 5% FCS, 37C, %5 CO2, 24h), I measured cytokine releasing
>with ELISA.
>Results: IL-2, IFNg: releasing. IL-4, TNFa, IL1a, IL1b: no releasing.
>I mean, this results could suggest Pa is presented and induce cytokine
>releasing by Th1 Tcell. But monocytes, the only APC available into human
>PBLs, appear not to be activated.
>I thought APC activation (in that cytokine releasing) and presentation were
>always together. But seeing my results my idea seems to be wrong.
>Could anyone tell me any opinion?
>>Thanks.
>Elena
>ervazquez at mail1.cti.unav.es
1) Pa might be crossing-linking receptors on the T cells.
2) It might be behaving as a superantigen.
Suggestion: try assaying nitrite production using the Greiss reaction
(indirect evaluation of nitric oxide production) on your plate reader.
The monocytes may be affected even if they don't produce Il-1.
Ed
