Padma, we are doing phagocytosis on human neutrophils using a FACscan. We
extract them from whole blood by centrifugation over mony-poly resolving medium
and histopaque, add autologous serum after washing and lysing, and highly
fluorescent beads. After an hour in a heated shaker-waterbath, we wash again
and run. Whole procedure takes about 2-3 hours. If you're interested, e-mail
me and I'll send the details. Kimi.
In article <CKIEtr.325 at mentor.cc.purdue.edu>, narayana at vet.vet.purdue.edu
(Padmakumar Narayanan) writes:
>Hai nettors,
>Can anyone out there furnish me with a successfull protocol for neutrophil phagocytosis assay. I PREFER FLOW CYTOMETRY BASED ASSAYS.
>THANKS A TON,
>PADMA KUMAR NARAYANAN
>PURDUE CYTOMETRY LABS
>SCHOOL OF VETERINARY MEDICINE
>PURDUE UNIVERSITY
>317-494-8638; FAX-317-494-0517
>
