In article <2isd48INN3q6 at owl.csrv.uidaho.edu>,
magri892 at crow.csrv.uidaho.edu (Magrini Vincent J.) wrote:
>> Does anyone know the pros and cons of producing mAb for PV sabin strains
> using hybridomas vs ascites fluid? Any input would a great help.
What will you use the antibody for?
IMHO, if you are using it for simple immunological procedures, FACS,
immunoprecipatation etc., ascites is wonderful. Mg/ml quatities of ab are
possible. The only cons I know of are that it can have various
contaminates from the host. However, one pass with lipoclear and then
affinity chromatography of some sort (protein A or G for IgG) yields very
pure ab. It clearly causes the mice pain but I know of no better way to
generate large quantities of antibody.
Allen Black
Univ. of Alabama at Birmingham
Dept. of Microbiology
