I was wondering if anyone knew of a sure fire way to isolate *healthy*
canine neutrophils from whole blood. I've tried Ficoll to localize the PMN's
in the RBC layer and then a series of hypotonic lysis to remove the RBC's.
I've also tried metrizamide (like ficoll but supposed to isolate
granulocytes) centrifugation with no success. There is no procedure, or I am
unable to find it. Any suggestions would be greatly appreciated.
Matt Sylte
Graduate Student: Pathology and Microbiology
College of Veterinary Medicine, Kansas State University
