IUBio

D-J recombination probes

txpljfg at UABCVSR.CVSR.UAB.EDU txpljfg at UABCVSR.CVSR.UAB.EDU
Thu Jul 7 11:53:56 EST 1994


Which locus?  Ig mu, TCR beta , or TCR delta?



> Dear immunonetters,
> 
> I am searching for oligo sequences to use as probes of V-D recombination.
> 
> Are there "universal primers" that can be used in PCR for all Ds and Js ?
> 
> Are there sequences that can be used to probe both human or mouse ?
> 
> Please send replies either to the net or directly to me and I wll post a
> summary. All replies are greatly appreciated.
> 
> Thanx a million
> 
> Uri
> Uri at pharma1.pharma.mcgill.ca
> 
> IT IS NEVER THE HYPOTHESIS WHICH IS WRONG, IT IS THE DATA (a theoretician)
> 

==============================================================================
James F. George, Ph.D.              "Back off man, I'm a scientist"
Department of Surgery                --Bill Murray
University of Alabama at Birmingham
205-934-4261 voice
txpljfg at uabcvsr.cvsr.uab.edu
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