Please excuse my ignorance but could someone tell me what are the differences
(by which I mean sensitivity, specificity etc. rather than the mechanics)
between developing an ELISA vs. RIA. I have noted that there appear to be more
commercial kits for RIA available, certainly from Peninsula, but my lab would
prefer to avoid Radioisotopes. If there is an RIA kit commercially available
is it possible to pocket the serum and develop an ELISA?
I have recently been supplied with a rabbit polyclonal serum and need
to set up a quantitative assay from scratch. Would it be as easy to set up an
ELISA than an RIA.
Excuse my ignorance again I am just a poor molecular biologist!
