I am working on the preparation of salivary glands mononuclear cell
suspension. After a 60 minutes collagenase (type II) digestion we use a
cell strainer. However we cannot obtain a suspension clean enough to use
gradient density separation (percoll or lympholyte M). We have problems
with cell clumping. Does anyone have a solution.
thank you.
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Jean Barbeau E-mail: barbeauj at medent.umontreal.ca Fax: (514) 343-2233
Tel: (514) 343-2366 Local: D-223
Faculte de medecine dentaire
Universite de Montreal
C.P. 6128, Succ. A,
Montreal, Quebec, Canada, H3C 3J7
