Perhaps it is important how you freeze them. Anecdotal information
suggests that a quick freeze in dry ice/ispropanol results in greater
retention of activity post thawing, i.e. that the damage occurs during
the freezing process.
nathan
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Nathan O. Siemers, Ph.D.
Bristol-Myers Squibb Pharmaceutical Research Institute
3005 First Avenue, Seattle, WA 98121
(206) 727-3741 siemers at bms.com