Has anyone an explanation to the following regular problem with thymus
cells fixed with methanol and prepared for detection of bromodeoxyuridine
by immunofluorescence. The technique works fine all the time except
during the summer months July, August, September. This has occurred for
several years now. That I suspect methanol is due to the fact that
another fixative in these cases may replace methanol and with excellent
results. I have considered the possibility that temperature, humidity or
air pressure may be different during this time of year, but did not find
any correlation. We have both hot and dry as well as cold and wet periods
during summer.
Goran
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Goran Sandberg tel: Int + 46 8 7287039
Dept Neuroscience fax: Int + 46 8 370757
KAROLINSKA INSTITUTET e-mail: Goran.Sandberg at neuro.ki.se
S-171 77 Stockholm, Sweden
