In message <94114105853.~INN-AGEa00163.bionet-news at dl.ac.uk>,
lolis at ELIRIS.MED.YALE.EDU (elias lolis) wrote:
>Does anyone have any strong opinions on what the most effective apparatus is
>for doing neutrophil chemotaxis assays? The Boyden-chamber seems to be
>heavily used but there appear to be other systems that do the job.
>Specifically,
>does anyone know the relative advantages/disadvantages of using the 48-well
>microchamber from Neuroprobe or any of the products sold by Costar. I am
>a protein biochemist who will shortly set up to do these chemotaxis assays
>(I've never done them before) and any help or references would be appreciated.
>>Elias
The chemotaxis assay under agarose is a simple method for neutrophil
chemotaxis wich is inexpensive and which can be easily set up with
materials found in any lab.
References:
J. Immunol. Meth. (1978) Orr W. and Ward P.A. vol. 20, p.95-107
Methods in Enzymology vol. 162 (Academic Press) (1988) Nelson R.D. and
Herron M.J. p.50
F. Beauvais
beauvai at World-net.sct.fr
Francis BEAUVAIS (beauvai at World-net.sct.fr)
