lw75 writes:
> From BIOSCI-REQUEST at net.bio.net Thu Nov 17 14:45:24 1994
> To: immunology at net.bio.net> From: Lynne_A_WHITEHEAD at umail.umd.edu (lw75)
> Subject: s-IgA
> Date: 17 Nov 1994 12:22:34 -0800
> Sender: daemon at net.bio.net> Message-ID: <9411172016.AA12915 at umailsrv1.UMD.EDU>
> NNTP-Posting-Host: net.bio.net
>> Hi there!
>> I am wondering if anyone can answer a question I have regarding the use of
> clinically isolated human secretory IgA in an eliza. I want to know if the
> secretory component of the antibody will inhibit binding of the secondary
> antibody (peroxidase labeled goat IgG anti human IgA) and/or the development
> of the eliza. Any other information regarding the use of s-IgA in
> lab protocols that may cause it to react differently than "normal"
> antibodies would be appreciated!
I never worked with IgA . According to this format (direct competitive),
your IgA are simply the polymerised antigens, no different than other
poly-identical subunit protein, therefore IgG here are not secondary but
first antibodies with label. You are detecting IgA but not antigen of IgA.
Why react abnormally? If you are informed with any reasons, please let me
know too. I really want to known. Sincerely
> Thanks in advance for any help!
>> Lynne Whitehead
> U of Maryland - Microbiology Dept.
>>>
