In article <3aj0jl$7e5 at jhunix1.hcf.jhu.edu>,
alafwalk at welchlink.welch.jhu.edu (Anne Lafond-Walker) wrote:
> Hi everyone,
> Does anyone know of any other kit to use that locates fragmented
> DNA in tissue sections other than ONCOR's Apoptag? This kit works
> fine but, is expensive and the amount of reagents you receive is
> small.
> Thanks for any help
> Anne
You might want to look up TUNEL staining by Gavrieli et al. in J. Cell
Biol. 119:493 (1992). I haven't used this myself, just saw the results
displayed recently in a seminar by Jonathan Sprent here at the NIH.
Beautiful. He said that a trick is to leave the slides in the coldroom for
a couple of days with (after?) the stain. The downside: it is not a kit.
You make up the reagents. The upside: probably much cheaper than a kit.
Good luck!
--
Rachel R. Caspi, Ph.D. phone: 301-496-6409
Laboratory of Immunology, NEI 301-496-6394
NIH Building 10, Room 10N222 fax: 301-402-0485
Bethesda, Maryland 20892 email: rcaspi at helix.nih.gov
