We are working on a project to raise antibodies to synthetic peptides using MAPs. We have synthesized a 15 residue peptide on a 4-branch MAP construct. This was used to immunize Balb/c mice and we obtained no antigenic response. We think this may be due to a lack of a T-cell helper epitope. Does anyone know of a universal T-cell epitope sequence compatible with Balb/c mice? Or, have another explaination for why we obtained no response?
Anne deVaux & Jacqueline McCandless
devauxae at ccmail.apldbio.com