try using mhc mismatached cells as well as a variety of other mabs.
W6/32 binds to the wrong side of class I.
On Tue, 29 Nov 1994, John Ferbas wrote:
> I have used an anti-MHC class I antibody (W632) in an attempt to demonstrate
> MHC class I restriction in 2 CD8+ CTL clones, but have had no success. I know
> that the antibody is saturating the targets by FACS analysis. Also, the
> antibody is added before the assay and present throughout. Is there any
> alternative way to demonstrate class I restriction? Your comments are greatly
> appreciated.
> ===========================================================================================
> John Ferbas Ph.D. Ferbas at cyto.medsch.ucla.edu> UCLA MED/Cellular Immunology & Cytometry Voice: (310) 825 6340
> 12-236 Factor Bldg. FAX: (310) 794 2145
> 10833 Le Conte Ave.
> Los Angeles CA 90024-1745
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