I have used an anti-MHC class I antibody (W632) in an attempt to demonstrate
MHC class I restriction in 2 CD8+ CTL clones, but have had no success. I know
that the antibody is saturating the targets by FACS analysis. Also, the
antibody is added before the assay and present throughout. Is there any
alternative way to demonstrate class I restriction? Your comments are greatly
appreciated.
===========================================================================================
John Ferbas Ph.D. Ferbas at cyto.medsch.ucla.edu
UCLA MED/Cellular Immunology & Cytometry Voice: (310) 825 6340
12-236 Factor Bldg. FAX: (310) 794 2145
10833 Le Conte Ave.
Los Angeles CA 90024-1745
