Greetings, everyone,
Using a flow cytometer and an antibody at various dilutions, I've
just analyzed the fluorescence intensity of a particular cell-antibody
combination. I now have a nice, asymptotic curve.
Mean
ug MAb added Fluorescence
----------------------------
0.00 8
0.63 364
1.25 541
2.50 812
5.00 1050
Now what? At what level is an antibody considered to be "titered"?
Any advice or references would be greatly appreciated. Thanks!
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Unique ID : Ladasky, John Joseph Jr.
Title : BA Biochemistry, U.C. Berkeley, 1989
Location : Stanford University Dept. of Cell Biology, Fairchild D-105
Keywords : immunology, music, running, Green
