Problem: too much nonspecific binding during Eliza of Human Serum Albumin
(HSA) in hepatocyte culture.
I am using the HSA MoAfrom sigma , the secondary is a peroxidase
conjugated IgG. I have far FAR to much nonspecific binding of the primary
anti HSA MoA to the plastic (or maybe the conc ratio between
antigen/primary , or btw primary and secondary is not the right one...
Any help ?
I can give more details of the experimental approach for those
able/willing to post their experience.
Any one out there working on HSA quantitation by ELIZA?
